![]() Insects may provide a possible solution as an alternative feed, since they can partially replace traditional feed sources, while they also provide a means to bio-converting organic waste ( 2). ![]() With the livestock industry at its limit in terms of sustainable production capacity, and the pet food business in constant growth, new sources of protein are being sought in order to meet the market's demand and the expectations of pet owners ( 1). Moreover, the study showed that both the in vivo marker method and the in vitro method could be possible alternatives to the traditional total collection method in digestibility trials. Digestibility analysis of a dog food containing insect meal as the sole source of protein (36.5% inclusion) showed promising results in terms of it presenting similar values as a meat-based diet, indicating its suitability as a sustainable protein source for pet food. The in vitro digestibility values for dry matter, organic matter, and crude protein, as well as the estimated in vivo digestibility of organic matter and crude protein by the means of the predictive equation, were aligned with the in vivo results, although in vitro estimations were consistently higher compared with those obtained by in vivo analysis. There was no difference between total collection and internal marker methods in the assessment of in vivo digestibility for any of the nutrients considered. On the contrary, fiber digestibility was lower in the insect-based diet compared with the venison diet ( p < 0.001). Calcium digestibility was higher in the BSF diet compared with the CTRL diet ( p = 0.018). However, a statistical trend ( p = 0.066) was observed indicating greater protein digestibility in the BSF diet compared with the CTRL diet. The two diets showed similar nutrient digestibility values for dry matter, organic matter, ether extract, ash, and phosphorus. The digestibility of nutrients was determined using both in vivo (“total collection” and “internal marker” approaches) and in vitro methods. ![]() ![]() Two isonitrogenous and isoenergetic dry diets containing either venison meal (CTRL diet) or black soldier fly larvae meal (BSF diet) as their primary sources of proteins were fed to six adult dogs, according to a Latin square design. Moreover, the purpose of the study was to compare the traditional in vivo total collection method with the in vivo marker method and in vitro digestibility method. The aim of the study was to evaluate nutrient digestibility of a diet containing black soldier fly larvae as its main protein source. Growing attention is being directed toward insects as a novel and sustainable source of protein for pet food. ![]()
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